Immunohistochemistry, or IHC for short, is a specialized lab test that allows us to examine specific proteins within cells and tissues. It combines the principles of immunology with histology, enabling us to visualize and identify these proteins under a microscope.
Immunohistochemistry (IHC) is a valuable diagnostic test used in the field of pathology. This test involves the use of antibodies to detect specific proteins in tissue samples. By staining these proteins, IHC helps pathologists identify and classify different types of cells or tumors.
One major use of Immunohistochemistry is in cancer diagnosis and prognosis. It allows doctors to determine the origin and behavior of abnormal cells, helping them tailor appropriate treatment plans for patients.
A positive test results in the discovery of a marker or receptor on the cell during the biopsy or denotes a specific alteration in the cancer protein. The good outcome suggests that the subject may have inherited a genetic disorder.
One common application of IHC is in determining the subtype of breast cancer. By analyzing the expression levels of certain markers, such as estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2/neu), pathologists can classify breast tumors into different subtypes – luminal A, luminal B, HER2-positive, or triple-negative.
IHC also plays a significant role in diagnosing lung cancer.
Furthermore, IHC assists in identifying other types of cancers like colorectal carcinoma, melanoma, lymphomas, and many more.
There are two main types of immunohistochemistry tests: direct and indirect methods.
The direct method involves the use of a primary antibody that directly binds to the target antigen in the tissue sample. This bound antibody is then visualized using a secondary antibody labeled with a detectable marker, such as an enzyme or fluorescent dye. The presence of this marker indicates the location of the target antigen within the tissue.
On the other hand, the indirect method amplifies the signal by utilizing an additional step. In this approach, an unlabeled primary antibody is first applied to bind with the target antigen. Subsequently, a secondary antibody labeled with a detectable marker is introduced to bind specifically with the primary antibody.
Yes, Fasting is required for an immunohistochemistry test.
Yes, home sample collection is available for immunohistochemistry panel 3 test. One can opt for home collection while booking a test through Max Lab.
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