What are the two main types of HPLC?

There are two main types of HPLC: normal-phase and reverse-phase. Normal-phase - Normal-phase HPLC uses a polar stationary phase, such as silica gel or alumina, and a non-polar mobile phase, such as hexane or ethyl acetate. Reverse phase - Reverse-phase HPLC uses a non-polar stationary phase, such as C18 bonded silica gel or polystyrene-divinylbenzene copolymer particles, and a polar mobile phase, such as water with an organic modifier like acetonitrile or methanol.

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Haemoglobin HPLC,EDTA Test

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HbA1a
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HAEMOGLOBIN HPLC TEST SAMPLE REPORT

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Sample type :

Blood

Description - The high-performance liquid chromatography (HPLC) test is a vital analytical technique that is used to separate and identify different types of haemoglobin in human blood. This test is commonly used in the medical sector to establish the concentration and purity of a sample. Usually, a doctor recommends a Hb HPLC test to find out about any abnormal haemoglobin, such as sickle cell haemoglobin, beta-thalassemia, and alpha-thalassemia. In the Hb HPLC test, the ....Read More

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Frequently Asked Questions (FAQ's)

The Haemoglobin HPLC test, also known as High-Performance Liquid Chromatography, is a medical procedure used to identify and quantify different types of haemoglobin in the blood. Hemoglobin is a protein found in red blood cells responsible for carrying oxygen throughout the body.

There are two main types of HPLC: normal-phase and reverse-phase.

Normal-phase - Normal-phase HPLC uses a polar stationary phase, such as silica gel or alumina, and a non-polar mobile phase, such as hexane or ethyl acetate.
Reverse phase - Reverse-phase HPLC uses a non-polar stationary phase, such as C18 bonded silica gel or polystyrene-divinylbenzene copolymer particles, and a polar mobile phase, such as water with an organic modifier like acetonitrile or methanol.

The Hb HPLC test is done to measure the levels of different types of haemoglobin in the blood. It is a type of high-performance liquid chromatography that detects and quantifies abnormal haemoglobins such as sickle cell haemoglobin, beta-thalassemia, and alpha-thalassemia.

The amount of time required for an HPLC test depends on various factors, such as the type of analysis being performed and the complexity of the sample being tested. Generally, a typical HPLC run can take anywhere from 10 minutes to over an hour.

In the pharmaceutical industry, HPLC is used to analyze drug compounds, detect impurities and ensure product purity. HPLC can also be used to determine the concentration of active ingredients in drugs.

Several factors can affect the sensitivity of HPLC, including the type of column used, particle size, flow rate and detector response. The composition of mobile phase and injection volume can also have an effect on HPLC sensitivity.

The normal range for HPLC of haemoglobin varies depending on age, gender, and other factors. However, generally speaking, the normal range for adults is between 97-105% while children may have slightly higher ranges.

About This Test

The high-performance liquid chromatography (HPLC) test is a vital analytical technique that is used to separate and identify different types of haemoglobin in human blood. When is an HPLC Test Pres

The high-performance liquid chromatography (HPLC) test is a vital analytical technique that is used to separate and identify different types of haemoglobin in human blood.

When is an HPLC Test Prescribed?

An HPLC test is prescribed by a doctor if an individual is at high risk of blood-related diseases, such as anaemia, family history, prenatal screening, or newborns.

To detect any abnormalities in the haemoglobin level in the blood, such as sickle cell haemoglobin, beta-thalassemia, and alpha-thalassemia. This test not only identifies the abnormal haemoglobin level in the blood but also quantifies its level in the blood.
The Hb haemoglobin test is also suggested for carrier screening in the patient. This helps the individual know if they carry any abnormal haemoglobin genes that can be transferred to their children. It helps with genetic counselling and family planning.
This test is also suggested as a part of monitoring disease progression. It helps the medical practitioner know the effectiveness of medical treatment on the patient.
The Hb HPLC test is also done in pregnancy; a normal range indicates a healthy pregnancy. If the test report is not in the normal range, it indicates a haemoglobin disorder in the mother.
This test is also recommended as a treatment plan for other medical conditions, including diabetes.

During the HPLC EDTA test procedure, EDTA is added to the blood sample. This helps maintain the integrity of red blood cells and prevents blood clotting. This sample is then analysed with HPLC. During the test, different types of haemoglobin are separated based on size, charge, or both.

Max Lab offers HPLC analysis tests at an affordable cost to make healthcare accessible to everyone. You can check the HPLC blood test price online at the official website of Max Lab and book the test from there.

Time Required for HPLC EDTA Test Reports + Next Steps

Once you have booked the test and the phlebotomist has collected your blood sample, the sample is taken to the laboratory, where the test is performed. It usually takes 24-48 hours for the test report to be generated. You can download it from the website or collect a physical copy.

After receiving your HPLC test results, it is crucial to consult with a qualified healthcare professional to understand the implications of the findings. They suggest a proper line of treatment.

Queries about this test

#1 Haemoglobin HPLC EDTA test method

Ans: The method used for this test involves separating and analyzing the haemoglobin molecules using high-performance liquid chromatography (HPLC) technology.

#2 Haemoglobin HPLC EDTA test procedure

Ans: During the procedure, EDTA is added to the collected sample which helps prevent clotting and preserves red blood cells' integrity. After this, samples are analyzed with HPLC that separates different types of hemoglobin based on their size, charge or both.