Home > Procedure preparations > Indirect Coombs Test Procedure
The indirect Coombs test can help diagnose autoimmune diseases, such as Lupus, and also helps identify potential sensitivities and allergies. It’s an important diagnostic tool for all sorts of reasons, but how does it work? In this blog post, we will explore the steps involved in the indirect Coombs test procedure. We’ll explain what it is, how it works, and why it’s important for diagnosing certain conditions. Read on to learn more about the indirect Coombs test and its uses.
The indirect Coombs test can be used to determine if there are any antibodies present in the blood, and if so, how many. The test can also be used to identify the specific antibody that is present.
This test is typically used to diagnose anemia or to determine if a person has been exposed to a certain disease. The test can also be used to monitor the progress of a person's immune system.
The indirect Coombs test is usually performed when a person has an autoimmune disorder or when they are pregnant. The test is also used to check for compatibility between donors and recipients of blood transfusions.
The Indirect Coombs Test is performed by adding patient serum (the liquid part of the blood) to red blood cells from a donor that has been treated with a special antibody. If antibodies are present in the patient's serum, they will attach to the donor cells. The cells are then washed and incubated with a second antibody that is tagged with a fluorescent dye. This second antibody will bind to the first antibody-tagged cells, making them visible under a microscope.
The Indirect Coombs Test (ICT) is used to detect antibodies in the patient's serum that are capable of binding to red blood cells (RBCs). In order to prepare for this test, the patient should fast for at least 8 hours before the scheduled appointment. The patient should also avoid taking any medications that could interfere with the test results.
The indirect Coombs test results usually take about two weeks. However, it may take longer if the person being tested is taking certain medications, such as corticosteroids or immunosuppressants.
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